Study Report

Reference	Fukuo, Y.,2010 PMID: 20394784
Citation	Fukuo, Y., T. Kishi, et al. (2010). "Serotonin 6 receptor gene and mood disorders: case-control study and meta-analysis." Neurosci Res 67(3): 250-255.
Disease Type	Bipolar Disorder & Major Depressive Disorder
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	1007 BP patients, 447 MDD patients and 1753 controls
SNP/Region/Marker Size	5 SNPs
Predominant Ethnicity	Mongloid
Population	Japanese
Gender	493 males and 514 females in BD patients,211 males and 236 females in MDD patients and 843 males and 910 females in controls
Age Group	Adults : Mean age(SD)(year):47.9(14.2) in BD patients,48.7(16.1) in MDD patients and 44.2(15.0) in controls

Sample Diagnosis	DSM
Sample Status	The subjects in the association analysis were 1007 BP patients (709 patients with bipolar I disorder and 298 patients with bipolar II disorder), 447 MDD patients, and 1753 healthy controls. All subjects were unrelated to each other, ethnically Japanese, and lived in the central area of Japan. 238 patients were diagnosed according to DSM-IV criteria with the consensus of at least two experienced psychiatrists on the basis of unstructured interviews and a review of medical records, although 209 of the patients underwent the Mini-International Neuropsychiatric Interview (MINI). Of the 450 MDD patients, 261 were treated with fluvoxamine, sertraline or paroxetine. 1576 controls were also diagnosed according to DSM-IV criteria with the consensus of at least two experienced psychiatrists on the basis of unstructured interviews, although 46 or 131 controls underwentthe MINI or Structured Clinical Interview forDSM-IVdisorders (SCID-1), respectively.Nonehad severe medical complications such as liver cirrhosis, renal failure, heart failure or other Axis-I disorders according to DSM-IV. Controls included hospital staff and medical students.
Technique	genotyping
Statistical Method	Among the clinical characteristics of patients in this pharmacogenetic study, individual t-test and chi-square tests(Fisher's exact test when appropriate) were used to compare means and categorical proportions, respectively.
Result Summary	RESULTS: The control subjects and the two groups of patients were similar with respect to the genotype and allele distribution belonging to the two polymorphisms in the serotonin transporter gene for. An interaction between status (control, unipolar- or bipolar patient) and VNTR genotype regarding the number of platelet serotonin transporters was observed; unipolar patients with the genotype 12/10 had more platelet serotonin transporters than bipolar patients and controls with this genotype. No association related to the polymorphism was found in the promoter region of the serotonin transporter gene. CONCLUSION: An association was observed between the polymorphism in intron 2 of the serotonin transporter gene and the number of platelet serotonin transporters. Unipolar patients with a particular genotype had more platelet serotonin transporters than the corresponding controls and bipolar patients.