Study Report

Reference	Kishi, T., 2008 (b) PMID: 18804497
Citation	Kishi, T., T. Kitajima, et al. (2008). "Association analysis of nuclear receptor Rev-erb alpha gene (NR1D1) with mood disorders in the Japanese population." Neurosci Res 62(4): 211-215.
Disease Type	Bipolar Disorder & Major Depressive Disorder
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	147 bipolar patients (93 patients with bipolar I disorder and 54 patients with bipolar II disorder), 322 major depressive disorder patients and 360 controls
SNP/Region/Marker Size	3 SNPs
Predominant Ethnicity	Mongloid
Population	Japanese
Gender	BD patients (75 males and 72 females); MDD patients (156 males and 166 females); healthy controls (201 males and 159 females)
Age Group	adults : BD patients: males mean age=47.8 (SD=14.6) years; MDD patients: males mean age=47.1 (SD=15.9) years; healthy controls: males mean age=35.9 (SD=14.7) years,

Sample Diagnosis	DSM-IV
Technique	genotyping
Statistical Method	Genotype deviation from the Hardy-Weinberg equilibrium (HWE) was evaluated by chi-square test (SAS/Genetics, release 8.2, SAS Japan Inc., Tokyo,Japan). Marker-trait association analysis was used to evaluate allele- and genotype-wise association with the chi-square test (SAS/Genetics, release 8.2, SAS Japan Inc.,Tokyo, Japan), and haplotype-wise association analysis was evaluated with a likelihood ratio test using the COCAPHASE2.403 program (Dudbridge, 2003). Bonferroni's correction was used to control inflation of the type I error rate. Power calculation was performed using two statistical programs prepared by Ohashi et al. (2001) (Ohashi et al.'s software) or Purcell et al. (2003) (Genetic Power Calculator: GPC). The significance level for all statistical tests was 0.05.
Result Summary	One SNP showed an association with bipolar disorder in females. After Bonferroni correction for multiple testing, however, this significance disappeared. No significant association was found withmajor depressive disorder.