Study Report

Basic Info
Reference |
Meira-Lima, I.,2003(a) PMID: 12895207
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Citation |
Meira-Lima, I., D. Jardim, et al. (2003). "Allelic association study between phospholipase A2 genes and bipolar affective disorder." Bipolar Disord 5(4): 295-299.
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Disease Type |
Bipolar Disorder |
Study Design |
case-control |
Study Type |
Candidate-gene association study |
Sample Size |
181 unrelated BPAD subjects and 312 controls |
SNP/Region/Marker Size |
3 variants |
Predominant Ethnicity |
|
Population |
Brazilian |
Gender |
Male/female:69/112 for BPAD patients and 146/166 for controls |
Age Group |
Adults
:
Mean age(SD)(year):43(12),21.8(7) at onset for BPAD patients and 36(9) for controls
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Detail Info
Sample Diagnosis |
DSM |
Sample Status |
BPAD patients. A total of 181 unrelated patients with BPAD aged between 18 and 80 years were recruited from public psychiatric assistance services in the Sao Paulo city metropolitan area. All patients who fulfilled the DSMIIIR criteria for BPAD were selected for the study. All patients had at least one manic episode, and the mean number of episodes was eight, 79% of the patients had a history of psychotic symptoms.Venous blood samples were collected after written informed consent was obtained. Controls. A total of 312 control subjects aged between 18 and 60 years were selected from unrelated subjects admitted to the Blood Donation Center of the Fundac?a?o Pro_ -Sangue ?Sao Paulo University Medical School, after an interview to exclude those with a personal or family history of mental illness. An attempt to match both groups according to ethnicity was based on phenotypic characteristics (pigmentation of the abdomen, hair color and type and conformation or the nose and lips) and known family origin. The ethnic distribution of both groups was as follows: 60% Caucasian, 34% mulatto and 6% black. |
Technique |
PCR,RFLP and electrophoresis |
Statistical Method |
Allelic and genotypic frequencies of polymorphism on cPLA2 and iPLA2 genes were analyzed among BPAD patients and the control group using the chi-square test (statistical software Epi-Info version 6.0, Atlanta, GA, USA). The distribution of repeat marker alleles on the sPLA2 gene was compared between patients and controls using the CLUMP program (London,UK). |
Result Summary |
There was no significant difference observed in the allelic and genotypic distribution between the BPAD and control groups for cPLA2 (genotype: chi2 = 0.8, 2df, p = 0.6; allele chi2 = 0, 1df, p = 0.9), iPLA2 (genotype: chi2 = 1.7, 2df, p = 0.4; allele: chi2 = 0.3, 1df, p = 0.6), and sPLA2 (allele: chi2 = 3.6, 6df, p = 0.8).CONCLUSION: Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increased risk for BPAD in our sample. |

Other variants reported by this study for BD (count: 3)
Variant Name |
Related Gene |
Type |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result Category |
iPLA2 C/T |
PLA2G6 |
point mutation |
C/T |
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Association analysis:chi square test, genotype: X2=1.7, 2df, p?0.4; allele: X2=0.3, 1df, P-value = 0.6).
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No significant association was observed in BD.
No significant association was observed in BD.
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Negative
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cPLA2 BanI dimorphic site |
KAT5 |
point mutation |
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Association analysis:chi square test, genotype: X2=0.8, 2df, P-value = 0.6; allele X2=0.0, 1df, P-value = 0.9
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No significant association was observed in BD.
No significant association was observed in BD.
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Negative
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PLA2G10 (AAT)n |
PLA2G10 |
microsatellite |
multi-allelic repeats |
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Association analysis: X2=4.7, 6df, P-value = 0.58
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No significant association was observed in BD.
No significant association was observed in BD.
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Negative
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Genes reported by this study for BD (count: 3)
Gene |
Statistical Values/Author Comments |
Result Category |
PLA2G6 |
Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increa......
Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increased risk for BPAD in our sample.
More...
|
Negative
|
PLA2G1B |
Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increa......
Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increased risk for BPAD in our sample.
More...
|
Negative
|
KAT5 |
Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increa......
Our results failed to demonstrate that the studied PLA2 polymorphisms were associated with an increased risk for BPAD in our sample.
More...
|
Negative
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