Study Report

Reference	Okuda, A.,2010 PMID: 19760522
Citation	Okuda, A., T. Kishi, et al. (2010). "Translin-associated factor X gene (TSNAX) may be associated with female major depressive disorder in the Japanese population." Neuromolecular Med 12(1): 78-85.
Disease Type	Bipolar Disorder & Major Depressive Disorder
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	314 MDD patients, 158 BP patients and 811 healthy controls
SNP/Region/Marker Size	3 SNPs
Predominant Ethnicity	Mongloid
Population	Japanese
Gender	155 males and 159 females in MDD patients,81 males and 77 females in BP patients,352 males and 459 females in healthy controls
Age Group	Adults : Mean age(SD)(year):37.2(15.9) for controls,47.3(14.9) for MDD patients and 47.9(14.2) for BD patients

Sample Diagnosis	DSM
Sample Status	The subjects in the association analysis were 314 MDD patients, 158 BP patients (99 patients with bipolar I disorder and 59 patients with bipolar II disorder), and 811 healthy controls. Of the 314 MDD patients, 120 (59 males and 61 females; 42.0,SD=17.2 years) were treated with fluvoxamine and diagnosed according to DSM-IV criteria with the consensus of at least two experienced psychiatrists on the basis of a review of medical records and assessments with the Structured Interview Guide for Hamilton Rating Scale for Depression (SIGH-D). The remaining MDD patients were diagnosed according to DSM-IV criteria with the consensus of at least two experienced psychiatrists on the basis of unstructured interviews and a review of medical records. Fluvoxamine was taken two or three times a day for 8 weeks. The initial total dose was 50-100 mg per day, and the dosage was then increased gradually to a maximum of 150 mg, depending on the patients' condition. Patients with insomnia and severe anxiety were prescribed benzodiazepine drugs, but no other psychotropic drugs were permitted during the study. All subjects were unrelated to each other, ethnically Japanese, and lived in the central area of Japan. All healthy controls were also psychiatrically screened based on unstructured interviews. None had severe medical complications such as cirrhosis, renal failure, heart failure, or other Axis-I disorders according to DSM-IV.
Technique	PCR and sequencing
Statistical Method	Marker-trait association analysis was used to evaluate allele- and genotype association with the Chi-square test (SAS/ Genetics, release 8.2, SAS Japan Inc, Tokyo, Japan), and haplotype association analysis was evaluated with a likelihood ratio test using the COCAPHASE2.403 program(Dudbridge 2003).
Result Summary	Sklar, P., S. B. Gabriel, et al. (2002). Family-based association study of 76 candidate genes in bipolar disorder: BDNF is a potential risk locus. Brain-derived neutrophic factor. Mol Psychiatry 7(6): 579-593.