Study Report

Reference	Okumura, T., 2010 PMID: 20016223
Citation	Okumura, T., T. Kishi, et al. (2010). "Genetic association analysis of functional polymorphisms in neuronal nitric oxide synthase 1 gene (NOS1) and mood disorders and fluvoxamine response in major depressive disorder in the Japanese population." Neuropsychobiology 61(2): 57-63.
Disease Type	Bipolar Disorder & Major Depressive Disorder
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	325 MDD patients, 154 BP patients and 807 controls
SNP/Region/Marker Size	1 SNP
Predominant Ethnicity	Mongloid
Population	Japanese
Gender	160 males and 165 females for MDD patients, 80 males and 74 females for BP patients, 354 males and 453 females for controls.
Age Group	adult : mean age(SD) = 47.3(14.9) years for MDD patients, 47.9(14.2) years for bipolar disorder patients and 37.2(15.9) years for controls.

Sample Diagnosis	DSM-IV
Sample Status	117 (58 males and 59 females; mean age (standard deviation) = 44.8 (16.7) years) MDD patients were treated with fluvoxamine. Patients with insomnia and severe anxiety were prescribed benzodiazepine drugs, but no other psychotropic drugs were permitted during the study.
Technique	We used TaqMan assays (Applied Biosystems, Foster City, Calif., USA) for all single nucleotide polymorphisms (SNPs).
Statistical Method	Genotype deviation from the Hardy-Weinberg equilibrium (HWE) was evaluated by the X2 test. Marker-trait association analysis was used to evaluate the allele- and genotype-wise association with the X2 test. Gender, age at the time of recruitment, fluvoxamine dose at 8 weeks and SIGH-D total score at baseline were covariates used in the analysis to better model the effect of genotype on the percent decrease in the SIGH-D score from baseline to the end of the period of observation. Power calculation was performed using the genetic power calculator.
Result Summary	We did not detect a significant association between NOS1 and MDD, BP or the fluvoxamine therapeutic response in MDD in allele/genotype-wise analysis