Study Report

Reference	Toyota, T.,2000 PMID: 11035444
Citation	Toyota, T., A. Watanabe, et al. (2000). "Association study on the DUSP6 gene, an affective disorder candidate gene on 12q23, performed by using fluorescence resonance energy transfer-based melting curve analysis on the LightCycler." Mol Psychiatry 5(5): 489-494.
Disease Type	Bipolar Disorder & Major Depressive Disorder
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	unipolars (n = 132), bipolars (n = 122) and controls (n = 299)
SNP/Region/Marker Size	1 variant
Predominant Ethnicity	Mongloid
Population	Japanese
Gender	Male/female:52/80 in unipolar patients,70/52 in bipolar patients and 177/122 in controls
Age Group	Adults : Mean age(SD)(year):56(14) for males and 58(13) for females in unipolar patients,50(13) for males and 52(12) for females in bipolar patients and 46(10) for males and 45(15) for females in controls

Sample Diagnosis	DSM
Sample Status	About 30% of the unipolars had a single episode and the rest had recurrent episodes.Sixty per cent of the bipolars were diagnosed as bipolar I and 40% were bipolar II. Control subjects were recruited from hospital staff documented to be free of psychoses, and company employees who did not manifest psychiatric problems in brief interviews by psychiatrists.
Technique	PCR and RFLP
Statistical Method	Tests for Hardy-Weinberg equilibrium and allele comparisons were made by the use of X2 statistics with the significance level at 5% Differences of genotype distributions were assessed by the Monte Carlo method using CLUMP program.
Result Summary	No evidence for a significant disease-causing effect was found in Japanese unipolars (n = 132) and bipolars (n = 122), when compared with controls (n = 299). More importantly, this study demonstrates that melting curve analysis on the LightCycler is an accurate, rapid and robust method for discriminating genotypes from biallelic markers. This strategy has the potential for use in high throughput scanning for and genotyping of single nucleotide polymorphisms (SNPs).