Study Report

Reference	Gella, A.,2011 PMID: 21702894
Citation	Gella, A., M. Segura, et al. (2011). "Is Ankyrin a genetic risk factor for psychiatric phenotypes?" BMC Psychiatry 11: 103.
Disease Type	Bipolar Disorder & Schizophrenia & Major Depressive Disorder
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	a case-control sample including 920 patients with schizophrenia, 400 with bipolar affective disorder, 220 patients with unipolar depression and 480 healthy controls.
SNP/Region/Marker Size	3 SNPs
Predominant Ethnicity	Caucasian
Population	German
Gender	68% males in SZ patients,58% male in BD patients,61% males in unipolar depression patients and 59% males in control
Age Group	Adults : Average age at onset and average age at recruitment(year):26.5 and 41 in SZ patients,32 and 42.5 in BD patients,43 and 51 in unipolar depression patients and 29[average age at recruitment(year)] in control

Sample Diagnosis	ICD10
Sample Status	Index cases were recruited from the Department of Psychiatry, Psychosomatics and Psychotherapy at of the University of Wurzburg. The sample encompassed 920 cases with psychosis according to ICD10 for schizophrenia or related diseases including 182 cases with schizoaffective disorder (ICD10 F20-F25). 400 cases with bipolar disorder and 220 cases with unipolar depression (F32-F33). Sample was further subdivided according to Leonhard's classification systematic schizophrenias (n = 228), unsystematic schizophrenias (n = 635), cycloid psychosis (n = 309), manic depression (n = 284) and monopolar depression (n = 90) [17]. Diagnosis in differentiated psychopathology was made by repeated personal examinations of experienced psychiatrists (BP, MG, GS).The 480 volunteer control subjects were recruited from the blood donor centre at the University of Wurzburg. The preponderance of males in both samples avoided gender distortion in comparison of cases and controls. All subjects were unrelated and of German Caucasian descent. The Ethics Committee of the University of Wurzburg had approved the study, and written informed consent was obtained from all subjects.
Technique	genotyping
Statistical Method	Software FAMHAP was used to test for association. Hardy-Weinberg equilibrium (HWE) and pairwise standardized linkage disequilibrium (LD) were calculated with the program HAPLOVIEW. The software'stastical power calculator' was used analyzing power for association test.
Result Summary	RESULTS: We found no association of rs9804190 and rs10994336 with bipolar disorder, unipolar depression or schizophrenia. In contrast to previous findings rs10761482 was associated with bipolar disorder (p = 0.015) but not with schizophrenia or unipolar depression. We observed no association with disease entities according to Leonhard's classification. CONCLUSION: Our results support a specific genetic contribution of ANK3 to bipolar disorder though we failed to replicate findings for schizophrenia. We cannot confirm ANK3 as a common risk factor for different diseases.