Study Report
Basic Info
| Reference |
Minocci, D.,2011 PMID: 21658778
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| Citation |
Minocci, D., J. Massei, et al. (2011). "Genetic association between bipolar disorder and 524A>C (Leu133Ile) polymorphism of CNR2 gene, encoding for CB2 cannabinoid receptor." J Affect Disord 134(1-3): 427-430.
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| Disease Type |
Bipolar Disorder |
| Study Design |
case-control |
| Study Type |
Candidate-gene association study |
| Sample Size |
160 BD patients(69 with BDI,11 with BDII) and 80 control subjects |
| SNP/Region/Marker Size |
3 SNPs |
| Predominant Ethnicity |
Caucasian |
| Population |
Italian |
| Gender |
Male/female:53/27 in BD patients and 106/54 in controls |
| Age Group |
Adults
:
Mean age(SD)(year):42.5(12.15),25.3(6.6) at onset in BD patients and 38.0(7.5) in controls
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Detail Info
| Sample Diagnosis |
DSM |
| Sample Status |
BD patients were recruited between September 2009 and November 2010 from adult outpatients in treatment at the Psychiatric Clinic of the University of Pisa. The objectives and the protocol of the study were thoroughly explained to potential participants and 80 subjects signed a written consent and were submitted to a clinical interview, for diagnostic assessment, evaluated by the clinicians, using the Structured Clinical Interview for DSM-IV axis-I disorders (SCID-I/P) (First et al., 1995). Socio-demographic information and biological samples for DNA analysis were also collected. A total of 160 age and gender matched subjects (in a ratio 1:2 to BD patients) were selected as control group within a population sample of 366 subjects recruited among healthy donors. They gave their written consent as well. |
| Technique |
Allele Specific Oligonucleotide (ASO)-PCR and restriction fragment length polymorphism (RFLP) |
| Statistical Method |
The differences in allele frequencies between groups were tested for significance by Fisher's Exact test on 2_2 contingency tables.All analyses were performed with STATA software (StataCorpLP, College Station, Texas, USA). |
| Result Summary |
RESULTS: A statistically significant association was found between BD and the CNR2 524C>A; Leu133Ile (P(chi(2)) = 0.001; OR = 4.74; 95% C.I. = 2.52-10.50) while no statistically significant difference between BD and control group was observed for the other two SNPs. CONCLUSION: Though further investigations are necessary to confirm this data, our results suggest that CB2 cannabinoid receptor may play a role in BD. |
SNPs reported by this study for BD (count: 3)
| SNP |
Related Gene(s) |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result Category |
| rs41311993 |
CNR2
|
524C>A |
|
Fisher's Exact test: allele, OR (95%C.I.)=4.74 (2.30-9.78), P-value = 0.001
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Significant association was found .
Significant association was found .
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Positive
|
| rs2501432 |
CNR2
|
315A>G |
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Fisher's Exact test: allele, OR (95%C.I.)=0.80 (0.46-1.41), P-value = 0.15
|
No significant association was observed.
No significant association was observed.
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Negative
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| rs2229579 |
CNR2
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1073C>T |
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Fisher's Exact test: allele, OR (95%C.I.)=0.70 (0.39-1.24), P-value = 0.21
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No significant association was observed.
No significant association was observed.
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Negative
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Genes reported by this study for BD (count: 1)
| Gene |
Statistical Values/Author Comments |
Result Category |
| CNR2 |
Though further investigations are necessary to confirm this data, our results suggest that CB2 canna......
Though further investigations are necessary to confirm this data, our results suggest that CB2 cannabinoid receptor may play a role in BD.
More...
|
Positive
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