Study Report

Reference	Nyegaard, M., 2010 PMID: 19693800
Citation	Nyegaard, M., J. E. Severinsen, et al. (2010). "Support of association between BRD1 and both schizophrenia and bipolar affective disorder." Am J Med Genet B Neuropsychiatr Genet 153B(2): 582-591.
Disease Type	Bipolar Disorder & Schizophrenia
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	490 patients with BPD, 527 patients with SZ, and 601 control individuals for UK/DK sample; 506 bipolar patients and 510 controls for UCL sample.
SNP/Region/Marker Size	11 SNPs for UK/DK sample, 7 SNPs for UCL sample
Predominant Ethnicity	Caucasian
Population	Denmark and England, English

Sample Diagnosis	ICD-10-DCR and DSM-IV
Technique	Genotyping in the UK/DK sample was carried out using the Sequenom MassARRAY Genotyping system. For the UCL sample, genotyping was performed at the Broad Institute ofHarvard andMIT using the Affymetrix 500Karray.
Statistical Method	Data quality control and association analysis were performed using the open-source toolset PLINK. Single marker association analysis was performed using the Cochran-Armitage trend test. Correction for multiple testing was performed using the method proposed by Nyholt. The degree of LD in the CEU population and in the UK/DK sample was calculated with the program HaploView. Tests for genetic homogeneity across samples were performed by applying an exact test as implemented in Arlequin. Association analysis stratified for collection site was carried out using the Cochran-Mantel-Haenszel test.
Result Summary	In the UK/DK sample we genotyped 11 SNPs in the BRD1 region, of which six showed association with SZ (minimal single marker P-values of 0.0014). Haplotype analysis revealed specific risk as well as protective haplotypes with a minimal P-value of 0.0027. None of the 11 SNPs showed association with BPD. However, analyzing seven BRD1 SNPs obtained from the BPD GWA study, positive associations with BPD was observed with all markers (minimal P-value of 0.0014).